Quantifying the cooperative subunit action in a multimeric membrane receptor.
نویسندگان
چکیده
In multimeric membrane receptors the cooperative action of the subunits prevents exact knowledge about the operation and the interaction of the individual subunits. We propose a method that permits quantification of ligand binding to and activation effects of the individual binding sites in a multimeric membrane receptor. The power of this method is demonstrated by gaining detailed insight into the subunit action in olfactory cyclic nucleotide-gated CNGA2 ion channels.
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عنوان ژورنال:
- Scientific reports
دوره 6 شماره
صفحات -
تاریخ انتشار 2016